1Amalia Safitri, 2Hadi Sarosa, 3Danis Pertiwi
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v7-i02-49Google Scholar Download Pdf
ABSTRACT
Excessive exposure to ultraviolet light affects cell and tissue homeostasis which has a damaging effect on DNA characterized by the formation of reactive oxygen species (ROS). Inflammation due to UV rays is the main cause of oxidative stress in the skin. Healing of wounds caused by sunburn through a complex process consisting of inflammatory cell infiltration, cell proliferation, and tissue remodeling phases to restore normal skin integrity and function, rapid healing and epithelialization to prevent infection and minimize functional and aesthetic complications. The researchers aimed to see the effect of administering sapodilla leaf extract cream (Manilkara zapota) on IL-6 and TGF-β levels in Wistar rats with sunburn. Experimental study with a post test only control group design using a total of 30 mice as experimental animals, divided randomly into 4 treatment groups consisting of a control group, a placebo group, a sapodilla leaf cream group with a dose of 25% and a 50% cream group. Mouse skin tissue exposed to UV was analyzed using the ELISA method to determine the levels of IL-6 and TGF-β. There was no significant difference in the average IL-6 levels between treatment groups with the one way anova difference test, a p value of 0.25 (p0.05), there was a significant difference in the average TGF-β levels for each group with a p value of 0.005 (P0.05), Manilkara zapota leaf extract cream had a significant effect on TGF-β levels at a dose of 50%. Administration of Manilkara zapota leaf extract cream has a dose-dependent effect on IL-6 and TGF-β levels in skin tissue of mice exposed to UV.
KEYWORDS:Manilkara zapota extract cream, IL-6, TGF-β, UV
REFERENCES1) Bosch R, Philips N, Suárez-Pérez JA, et al. Mechanisms of photoaging and cutaneous photocarcinogenesis, and photoprotective strategies with phytochemicals. Antioxidants. 2015;4(2):248-268. doi:10.3390/antiox4020248.
2) Ke Y, Wang XJ. TGFβ Signaling in Photoaging and UV-Induced Skin Cancer. Journal of Investigative Dermatology. 2021;141(4):1104-1110. doi:10.1016/j.jid.2020.11.007.
3) Noorbakhsh SI, Bonar EM, Polinski R, Amin MS. Educational Case: Burn Injury—Pathophysiology, Classification, and Treatment. Acad Pathol. 2021;8:23742895211057240. doi:10.1177/237428952110572.
4) Ganguly A, Mahmud Z Al, Uddin MMN, Rahman SMA. In-vivo anti-inflammatory and anti-pyretic activities of Manilkara zapota leaves in albino Wistar rats. Asian Pac J Trop Dis. 2013;3(4):301-307. doi:10.1016/S2222-1808(13)60073-0.
5) Guerra KC, Crane JS. Sunburn. StatPearls [Internet]; 2022.
6) Markiewicz-Gospodarek A, Kozioł M, Tobiasz M, Baj J, Radzikowska-Büchner E, Przekora A. Burn Wound Healing: Clinical Complications, Medical Care, Treatment, and Dressing Types: The Current State of Knowledge for Clinical Practice. Int J Environ Res Public Health. 2022;19(3):1338. doi:10.3390/ijerph19031338.
7) Queirós CS, Freitas JP. Sun Exposure: Beyond the Risks. Dermatol Pract Concept. Published online October 31, 2019:249-252. doi:10.5826/dpc.0904a01.
8) Hung M, Beazer IR, Su S, Bounsanga J, Hon ES, Lipsky MS. An Exploration of the Use and Impact of Preventive Measures on Skin Cancer. Healthcare. 2022;10(4):743. doi:10.3390/healthcare10040.
9) Alrashood ST, Al-Asmari AK, Alotaibi AK, et al. Protective effect of lyophilized sapodilla (Manilkara zapota) fruit extract against CCl4-induced liver damage in rats. Saudi J Biol Sci. 2020;27(9):2373-2379. doi:10.1016/j.sjbs.2020.05.010.
10) Haghdoost F, Baradaran Mahdavi MM, Zolfaghari B, et al. The effect of Quercus brantii gall extract on burn wound healing in rat. Iran J Basic Med Sci. 2016;9(10):1144-1150.
11) Trinh XT, Long NV, Van Anh LT, et al. A Comprehensive Review of Natural Compounds for Wound Healing: Targeting Bioactivity Perspective. Int J Mol Sci. 2022;23(17):9573. doi:10.3390/ijms 23179573.
12) Karle PP, Dhawale SC, Navghare V V., Shivpuje SS. Optimization of extraction conditions and evaluation of Manilkara zapota (L.) P. Royen fruit peel extract for in vitro α-glucosidase enzyme inhibition and free radical scavenging potential. Futur J Pharm Sci. 2021;7(1):151. doi:10.1186/s43094-021-00305-4.
13) Puspitasari AD, Yuita NE, Sumantri S. KRIM ANTIOKSIDAN EKSTRAK ETANOL DAUN KOPI ARABIKA (Coffea Arabica). Jurnal Ilmiah Teknosains. 2017;3(2). doi:10.26877/jitek.v3i2.1884.
14) Prihardini, Wiyono AS. Pengembangan Dan Uji Antibakteri Ekstrak Daun Sawo Manila ( Manilkara Zapota ) Sebagai Lotio Terhadap Staphyllococcus aures The Development And Antibacteria Test Of Manila Sapodilla Leaf ( Manilkara Zapota ) AS A LOTIO TO Staphyllococcus aures. Jurnal Wiyata,. 2019;2(1):87-92.
15) Tanveer MA, Rashid H, Tasduq SA. Molecular basis of skin photoaging and therapeutic interventions by plant-derived natural product ingredients: A comprehensive review. Heliyon. 2023;9(3). doi:10.1016/j.heliyon.2023.e13580.
16) Ciążyńska M, Olejniczak-Staruch I, Sobolewska-Sztychny D, Narbutt J, Skibińska M, Lesiak A. Ultraviolet radiation and chronic inflammation-molecules and mechanisms involved in skin carcinogenesis: A narrative review. Life. 2021;11(4). doi:10.3390/life11040326.
17) MacLeod AS, Mansbridge JN. The Innate Immune System in Acute and Chronic Wounds. Adv Wound Care (New Rochelle). 2016;5(2):65-78. doi:10.1089/wound.2014.0608.
18) Larouche J, Sheoran S, Maruyama K, Martino MM. Immune regulation of skin wound healing: Mechanisms and novel therapeutic targets. Adv Wound Care (New Rochelle). 2018;7(7):209-231. doi:10.1089/wound.2017.0761.
19) Zhang T, Wang XF, Wang ZC, et al. Current potential therapeutic strategies targeting the TGF-β/Smad signaling pathway to attenuate keloid and hypertrophic scar formation. Biomedicine and Pharmacotherapy. 2020;129. doi:10.1016/j.biopha.2020.110287.
20) Ghafouri-Fard S, Askari A, Shoorei H, et al. Antioxidant therapy against TGF-β/SMAD pathway involved in organ fibrosis. J Cell Mol Med. Published online January 1, 2023. doi:10.1111/jcmm.18052.
Volume 07 Issue 02 February 2024
There is an Open Access article, distributed under the term of the Creative Commons Attribution – Non Commercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/), which permits remixing, adapting and building upon the work for non-commercial use, provided the original work is properly cited.
Our Services and Policies
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected.
The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.
International Journal of Multidisciplinary Research and Analysis will publish 12 monthly online issues per year,IJMRA publishes articles as soon as the final copy-edited version is approved. IJMRA publishes articles and review papers of all subjects area.
Open access is a mechanism by which research outputs are distributed online, Hybrid open access journals, contain a mixture of open access articles and closed access articles.
International Journal of Multidisciplinary Research and Analysis initiate a call for research paper for Volume 07 Issue 05 (May 2024).
PUBLICATION DATES:
1) Last Date of Submission : 26 May 2024 .
2) Article published within a week.
3) Submit Article : editor@ijmra.in or Online
Why with us
1 : IJMRA only accepts original and high quality research and technical papers.
2 : Paper will publish immediately in current issue after registration.
3 : Authors can download their full papers at any time with digital certificate.
The Editors reserve the right to reject papers without sending them out for review.
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected. The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.