1Nenny Lynda Caroline Hutabarat, 2Prasetyowati Subchan, 3Agung Putra
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v7-i02-46Google Scholar Download Pdf
ABSTRACT
UVB irradiation can induce the formation of Reactive Oxygen Species (ROS) which causes activation of melanin synthesis through activation of tyrosinase and tyrosinase related protein-1 (TRP1). Secondary metabolites contained in petai peel extract are known to play a role in inhibiting ROS production due to exposure to UVB rays. This study aims to determine the effect of administering petai peel extract gel on the expression of the tyrosinase and TRp1 genes in mouse skin tissue exposed to UVB. The research design was posttest only control group with a completely randomized design method. The samples studied were 24 mice exposed to UVB light with a wavelength of 302 nm and an energy of 390mJ/cm2/day 3 times a week for 2 weeks. This research was carried out in four groups, namely the healthy group (K1), the negative control group (K2), treatment 1 (K3) with 10% petai peel extract gel and treatment 2 (K4) with 20% petai peel extract gel. Tyrosinase and TRP1 gene expression was analyzed using qRT-PCR. qRT-PCR analysis showed that there was a significant decrease in tyrosinase and TRP1 gene expression between groups K3 (tyrosinase 3,19±2,12 and TRP1 4,96±3,42) and K4 (tyrosinase 0,65±0,44 and TRP1 2,22±1,18) compared to group K2 (tyrosinase 17,92±3,77 and TRP1 35,91±4,52). Administration of petai peel extract gel can reduce the expression of tyrosinase and TRP1 genes in hyperpigmentation mice exposed to UVB light.
KEYWORDS:UVB exposure, petai peel extract, tyrosinase, TRP1
REFERENCES1) Buechner N, Schroeder P, Jakob S, Kunze K, Maresch T, Calles C et al. Changes of MMP-1 and collagen type Iα1 by UVA, UVB and IRA are differentially regulated by Trx-1. Exp Gerontol 2008; 43: 633–637.
2) Wölfle U, Esser PR, Simon-Haarhaus B, Martin SF, Lademann J, Schempp CM. UVB-induced DNA damage, generation of reactive oxygen species, and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo. Free Radic Biol Med 2011; 50: 1081–1093.
3) Niu C, Aisa HA. Upregulation of Melanogenesis and Tyrosinase Activity: Potential Agents for Vitiligo. Molecules 2017; 22. doi:10.3390/molecules22081303.
4) Kim SS, Kim MJ, Choi YH, Kim BK, Kim KS, Park KJ et al. Down-regulation of tyrosinase, TRP-1, TRP-2 and MITF expressions by citrus press-cakes in murine B16 F10 melanoma. Asian Pac J Trop Biomed 2013; 3: 617–622.
5) Xue L, Li Y, Zhao B, Chen T, Dong Y, Fan R et al. TRP-2 mediates coat color pigmentation in sheep skin. Mol Med Rep 2018; 17: 5869–5877.
6) 6Hada M, Mondul AM, Weinstein SJ, Albanes D. Serum retinol and risk of overall and site-specific cancer in the ATBC study. Am J Epidemiol 2020; 189: 532–542.
7) Baliña LM, Graupe K. The Treatment of Melasma 20% Azelaic Acid versus 4% Hydroquinone Cream. Int J Dermatol 1991; 30: 893–895.
8) Rittié L, Fisher GJ. Natural and sun-induced aging of human skin. Cold Spring Harb Perspect Med 2015; 5: 1–14.
9) Bibbins-Domingo K, Grossman DC, Curry SJ, Davidson KW, Ebell M, Epling JW et al. Screening for skin cancer US preventive services task force recommendation statement. JAMA - Journal of the American Medical Association 2016; 316: 429–435.
10) Ibrahim N, Haluska FG. Molecular pathogenesis of cutaneous melanocytic neoplasms. Annual Review of Pathology: Mechanisms of Disease 2009; 4: 551–579.
11) Pérez-Cano FJ, Castell M. Flavonoids, inflammation and immune system. Nutrients 2016; 8: 8–11.
12) Andy S, Najatullah, Nugroho Trilaksana, Neni Susilaningsih. The Effect of Ethanolic Extract from Moringa oleifera Leaves in Collagen Density and Numbers of New Capillary Vessel Count on Wistar Rats Burn Wound. Bioscientia Medicina : Journal of Biomedicine and Translational Research 2022; 6: 1936–1941.
13) Siow HL, Gan CY. Extraction of antioxidative and antihypertensive bioactive peptides from Parkia speciosa seeds. Food Chem 2013; 141: 3435–3442.
14) Izzah Ahmad N, Abdul Rahman S, Leong Y-H, Azizul NH. A Review on the Phytochemicals of Parkia Speciosa, Stinky Beans as Potential Phytomedicine. J Food Sci Nutr Res 2019; 02. doi:10.26502/jfsnr.2642-11000017.
15) Tuerxuntayi A, Liu Y qiang, Tulake A, Kabas M, Eblimit A, Aisa HA. Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells. BMC Complement Altern Med 2014; 14: 1–9.
16) Mustafa NH, Ugusman A, Jalil J, Kamisah Y. Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells. J Appl Pharm Sci 2018; 8: 152–158.
17) Milac AL, Negroiu G. The Multiple Roles of Tyrosinase-Related Protein-2/L- Dopachrome Tautomerase in Melanoma: Biomarker, Therapeutic Target, and Molecular Driver in Tumor Progression. Human Skin Cancers - Pathways, Mechanisms, Targets and Treatments 2018. doi:10.5772/intechopen.70513.
18) Gui JS, Jalil J, Jubri Z, Kamisah Y. Parkia speciosa empty pod extract exerts anti-inflammatory properties by modulating NFκB and MAPK pathways in cardiomyocytes exposed to tumor necrosis factor-α. Cytotechnology 2019; 71: 79–89.
19) Zukhiroh Z, Putra A, Chodidjah C, Sumarawati T, Subchan P, Trisnadi S et al. Effect of Secretome-Hypoxia Mesenchymal Stem Cells on Regulating SOD and MMP-1 mRNA Expressions in Skin Hyperpigmentation Rats. Open Access Maced J Med Sci 2022; 10: 1–7.
20) Iqbal IY. PEMBERIAN KRIM EKSTRAK ETANOL BIJI PETAI (Parkia speciosa) 20% SAMA EFEKTIF DENGAN KRIM HIDROKUINON 4% DALAM MENGHAMBAT PEMBENTUKAN JUMLAH MELANIN PADA KULIT MARMUT (Cavia porcellus) YANG DIPAPAR SINAR ULTRAVIOLET B IRAH YUNITA IQBAL. Denpasar, Indonesia.
21) Freyhaus H Ten, Dagnell M, Leuchs M, Vantler M, Berghausen EM, Caglayan E et al. Hypoxia enhances platelet-derived growth factor signaling in the pulmonary vasculature by down-regulation of protein tyrosine phosphatases. Am J Respir Crit Care Med 2011. doi:10.1164/rccm.200911-1663OC.
22) Padhi S, Sarangi RL, Ramdas A, Ravichandran K, Varghese RGB, Alexander T et al. Cutaneous hyperpigmentation in megaloblastic anemia: A five year retrospective review. Mediterr J Hematol Infect Dis 2016; 8. doi:10.4084/MJHID.2016.021.
23) Rong J, Shan C, Liu S, Zheng H, Liu C, Liu M et al. Skin resistance to UVB-induced oxidative stress and hyperpigmentation by the topical use of Lactobacillus helveticus NS8-fermented milk supernatant. J Appl Microbiol 2017; 123: 511–523.
24) Li L, Ngo HTT, Hwang E, Wei X, Liu Y, Liu J et al. Conditioned medium from human adipose-derived mesenchymal stem cell culture prevents uvb-induced skin aging in human keratinocytes and dermal fibroblasts. Int J Mol Sci 2020; 21. doi:10.3390/ijms21010049.
25) Alam MB, Bajpai VK, Lee JI, Zhao P, Byeon JH, Ra JS et al. Inhibition of melanogenesis by jineol from Scolopendra subspinipes mutilans via MAP-Kinase mediated MITF downregulation and the proteasomal degradation of tyrosinase. Sci Rep 2017; 7: 1–12.
26) Khatib S, Nerya O, Musa R, Shmuel M, Tamir S, Vaya J. Chalcones as potent tyrosinase inhibitors: The importance of a 2,4-substituted resorcinol moiety. Bioorg Med Chem 2005; 13: 433–441.
27) Oh TI, Yun JM, Park EJ, Kim YS, Lee YM, Lim JH. Plumbagin suppresses α-MSH-induced melanogenesis in B16F10 mouse melanoma cells by inhibiting tyrosinase activity. Int J Mol Sci 2017; 18. doi:10.3390/ijms18020320.
28) Siebenga PS, van Amerongen G, Klaassen ES, de Kam ML, Rissmann R, Groeneveld GJ. The ultraviolet B inflammation model: Postinflammatory hyperpigmentation and validation of a reduced UVB exposure paradigm for inducing hyperalgesia in healthy subjects. European Journal of Pain (United Kingdom) 2019; 23: 874–883.
29) Lin MH, Hung CF, Sung HC, Yang SC, Yu HP, Fang JY. The bioactivities of resveratrol and its naturally occurring derivatives on skin. J Food Drug Anal 2021; 29: 15–38.
30) Lai X, Wichers HJ, Soler-Lopez M, Dijkstra BW. Structure of Human Tyrosinase Related Protein 1 Reveals a Binuclear Zinc Active Site Important for Melanogenesis. Angewandte Chemie - International Edition 2017; 56: 9812–9815.
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