1Ihdina Hanifa Hasanal Ibrahim,2Agung Putra,3Titiek Sumarawati
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v6-i9-75Google Scholar Download Pdf
ABSTRACT:
Diabetes mellitus (DM) type 2 is a metabolic disorder with the occurrence of insulin receptor resistance and reduced ability of pancreatic β-cells to secrete insulin, there will be an increase in adipose tissue which causes hypertrophy and hyperplasia. This situation triggers inflammation involving the role of pro-inflammatory cytokines and chemokines such as TNF-α (Tumor Necrosis Factor-Alpha) and MCP-1 (Monocyte Chemotactic Protein-1). Cell-based therapies such as the administration of hypoxic mesenchymal stem cell secretom offer a new paradigm in the management of T2DM. This study aims to determine the effect of hypoxic mesenchymal stem cell (SSPM) administration on TNF-α and MCP-1 gene expression in STZ-induced obese DM rats. This research is experimental with a post-test-only control group design approach. The subjects of the study were 20 male Wistar rats which were randomly divided into 4 groups. Group K1 was given standard feed and distilled water. Group K2 subjects with obesity mellitus type 2 with 0.9% NaCl. Groups K3 and K4, subjects with type 2 obesity mellitus, were treated with secretom doses of 250 μl and 500 μl. The study was conducted at the Sultan Agung Stem Cell Research Institute Laboratory for 21 days by taking blood for examination of TNF-α and MCP-1 gene expression using the PCR method. The result showed that the lowest average TNF expression was in the K4 group (3.19 pg/ml) and the lowest mean MCP-1 expression was in the K4 group (1.57 %). One-way ANOVA test showed a significant difference in TNF- expression with a value of p=0.006 and MCP1 expression showed a significant difference p=0.000 (p<0.05). The Administration of hypoxic mesenchymal stem cell (SSPM) secretom significantly reduced TNF-α and MCP-1 gene expression in STZ-induced obese DM rats.
KEYWORDS:Diabetes mellitus, TNF-α, MCP-1, SPPM
REFERENCES1) Anas Y, Rositasati R, Fitriani MR, Suharjono. Development of Type 2 Diabetic Mellitus Rat Experimental Animal Model due to Insulin Resistance Induced by Long Term Human Insulin. J Farm Science and Clinical Farm. 2015;12(2):16-23.
2) Akhavanpoor M, Wangler S, Gleissner CA, Korosoglou G, Katus HA, Erbel C. Adventitial inflammation and its interaction with intimal atherosclerotic lesions. Front Physiol. 2014;5 AUG(August):1-8. doi:10.3389/fphys.2014.00296
3) Yustisiani A, Andari D, Isbandiyah. The Effect of Giving Coffee on Reducing Blood Glucose Levels in Wistar Strain Diabetes Mellitus White Rats Type 2. Santika Med. 2013;9(1):38-45. doi:10.22219/sm.v9i1.4124
4) I Made Subhawa Harsa. THE EFFECT OF HIGH-FAT DIET ON BLOOD FAT PROFILE OF WHITE RATS (Rattus norvegicus). Doctoral Science. 2019;3(1):21-28.
5) Karussis D, Karageorgiou C, Vaknin-Dembinsky A, et al. Safety and immunological effects of mesenchymal stem cell transplantation in patients with multiple sclerosis and amyotrophic lateral sclerosis. Arch Neurol, 2010;67(10):1187-1194. doi: 10.1001/archneurol.2010.248
6) Sun H, Saeedi P, Karuranga S, Pinkepank M, Ogurtsova K, Duncan BB et al. IDF Diabetes Atlas: Global, regional, and country-level diabetes prevalence estimates for 2021 and projections for Diab Res Clin Pr. 2021.
7) Ministry of Health of the Republic of Indonesia. Stay Productive, Prevent and Overcome Diabetes Mellitus.; 2020.
8) PB. Perkeni.GUIDELINES FOR THE MANAGEMENT AND PREVENTION OF TYPE 2 DIABETES MELLITUS IN INDONESIA 2021.; 2019.
9) Ullah I, Subbarao RB RG. Human mesenchymal stem cells - Current trends and future prospective. Biosci Rep. 2015;35.
10) Miceli V, Bulati M, Iannolo G, Zito G, Gallo A, Conaldi PG. Therapeutic properties of mesenchymal stromal/stem cells: The need for cell priming for cell-free therapies in regenerative medicine. Int J Mol Sci. 2021;22(2):1-20. doi:10.3390/ijms22020763
11) Ribot J, Caliaperoumal G, Paquet J, Boisson-Vidal C, Petite H, Anagnostou F. Type 2 Diabetes Alters Mesenchymal Stem Cell Secretome composition and angiogenic properties. J Cell Mol Med. 2017;21(2):349-363. doi:10.1111/jcmm.12969
12) Item F. Visceral fat and metabolic inflammation: The portal theory revisited Visceral fat and metabolic inflammation: The portal theory revisited Division of Pediatric Endocrinology and Diabetology and Children Research’s Centre, University Children’s Hosp. 2012:13:30–9.
13) Rytka JM, Wueest S, Schoenle EJ, Konrad D. The Portal Theory Supported by Venous Drainage-Selective Fat Transplantation or/licenses/by-nc-nd/3.0/ for details. Diabetes. 2011; 60. doi:10.2337/db10-0697
Volume 06 Issue 09 September 2023
There is an Open Access article, distributed under the term of the Creative Commons Attribution – Non Commercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/), which permits remixing, adapting and building upon the work for non-commercial use, provided the original work is properly cited.
Our Services and Policies
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected.
The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.
International Journal of Multidisciplinary Research and Analysis will publish 12 monthly online issues per year,IJMRA publishes articles as soon as the final copy-edited version is approved. IJMRA publishes articles and review papers of all subjects area.
Open access is a mechanism by which research outputs are distributed online, Hybrid open access journals, contain a mixture of open access articles and closed access articles.
International Journal of Multidisciplinary Research and Analysis initiate a call for research paper for Volume 07 Issue 05 (May 2024).
PUBLICATION DATES:
1) Last Date of Submission : 26 May 2024 .
2) Article published within a week.
3) Submit Article : editor@ijmra.in or Online
Why with us
1 : IJMRA only accepts original and high quality research and technical papers.
2 : Paper will publish immediately in current issue after registration.
3 : Authors can download their full papers at any time with digital certificate.
The Editors reserve the right to reject papers without sending them out for review.
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected. The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.