1Ahmad Yassir,2Siti Thomas Zulaikhah, 2Minidian Fasitasari
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v6-i9-74Google Scholar Download Pdf
ABSTRACT:
Dyslipidemia is a lipid profile abnormality characterized by the increase of low-density lipoprotein (LDL), total cholesterol, triglycerides (TG), and a decrease of high-density lipoproteins (HDL). Dyslipidemia causes oxidative stress which can be shown by increasing levels of malondialdehyde MDA and decreased levels of antioxidants superoxide dismutase (SOD). This study aims to determine the effect of omega-3 fatty acids on MDA and SOD levels in dyslipidemic rat models. This study is experimental research with a post-test-only control group design. The research subject was 24 male Wistar rats were randomly divided into four groups, namely K0 (control), K1, K2, and K3. Dyslipidemia was induced by giving quail egg yolk 4ml/kg BW/day for 14 days and then followed by giving an omega-3 fatty acids dose of 36 mg/kg BW/day to the K2 group and dose of 72mg/kg BW/day to the K3 group for 14 days. The MDA statistical analysis used the One Way Anova and Post-hoc test and the SOD data analysis used the One-Way Anova and Tamnhane test. The MDA rate was higher in K2 compared to K1, and K3 with a value of p>0.05. SOD levels in K2 increased compared to K1 and K3 with a p>0.05. The administration of omega-3 fatty acids at doses of 36 mg/kg BW/day and 72 mg/kg BW/day for 14 days did not affect reducing MDA levels in dyslipidemic rats but the administration of omega-3 fatty acids at a dose of 36 mg/kg BW/day for 14 days increased SOD levels insignificantly.
KEYWORDS:Omega-3 fatty acids, MDA levels, SOD levels, dyslipidemia
REFERENCES1) Wahjuni S. Dyslipidemia Causes Oxidative Stress Marked by Increased Malondialdehyde. Denpasar: Udayana University Press; 2015.
2) Krisnansari D, Kartasurya MI, Rahfiludin MZ. Vitamin E Supplementation and Lipid Profile of Dyslipidemia Sufferers: A Study on Hospital Staff Professor Doctor Margono Soekarjo Purwokerto. Providing Electrolyte Carbohydrate Liquid, Hydration Status and Fatigue in Female Workers. 2012;46(1):6–11.
3) Berawi KN, Agverianti T. The effect of physical activity on the formation of free radicals as a risk factor for atherosclerosis. Majority Journal. 2017;6(2):86–91.
4) Tóth PP, Potter D, Ming EE. Prevalence of lipid abnormalities in the United States: The National Health and Nutrition Examination Survey 2003-2006. J Clin Lipidol. 2012;6(4):325–30.
5) Lin CF, Chang YH, Chien SC, Lin YH, Yeh HY. Epidemiology of Dyslipidemia in the Asia Pacific Region. Int J Gerontol. 2018;12(1):2–6.
6) PERKENI. Guidelines for the Management of Dyslipidemia in Indonesia 2019. PB Perkeni. 2019;9.
7) Dr. Ir. Sri Wahjuni MK. Dyslipidemia Causes Oxidative Stress Characterized by Malondialdehyde. Book. 2015;
8) Hartono RI, Simanjuntak K. The effectiveness of omega-3 supplementation on total cholesterol levels in alloxan-induced Wistas strain rats (Rattus norvegicus). Syiah Kuala Medical Journal. 2022;10(3):26–32.
9) Heshmati J, Morvaridzadeh M, Maroufizadeh S, Akbari A, Yavari M, Amirinejad A, et al. Omega-3 fatty acids supplementation and oxidative stress parameters: A systematic review and meta-analysis of clinical trials. Pharmacol Res. 2019;149:104462.
10) Fonda G, Pranata R, Deka H. Role of Omega-3 Fatty Acids in Dyslipidemia and Cardiovascular Diseases. Indonesian Journal of Cardiology •. 2016;37(4):213–35.
11) Lee KS, Chun SY, Kwon YS, Kim S, Nam KS. Deep sea water improves hypercholesterolemia and hepatic lipid accumulation through the regulation of hepatic lipid metabolic gene expression. Mol Med Rep. 2017;15(5):2814–22.
12) Mittal M, Siddiqui MR, Tran K, Reddy SP, Malik AB. Reactive oxygen species in inflammation and tissue injury. Antioxid Redox Signal. 2014;20(7):1126–67.
13) Forrester SJ, Kikuchi DS, Hernandes MS, Xu Q, Griendling KK. Reactive oxygen species in metabolic and inflammatory signaling. Circ Res. 2018;122(6):877–902.
14) G Morabito , P Kucan MS. Endocrine , Metabolic & Immune Disorders Drug Targets. JOURnal of food microbiology. 2015;15(1):1–2.
15) Lusiantari R, Pramaningtyas MD, Nurmasitoh T, Pattimura RH, Dewanti A. Shortening tends to increase aortic foam cell count and wall thickness in male Wistar rats. Universa Medicina. 2018;37(1):13.
16) Mulyanti. The Effect of Young Coconut Water (Cocos Nucifera L) on Parasitemia Index, Malondialdehyde Levels and Hemoglobin Levels in Malaria. Diponegoro University. Diponegoro University Semarang; 2016.
17) Diana FM. Omega 3. Agro Food Ind Hi Tech. 2012;17(1):29–31.
18) Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014.
19) Tsuyoshi Tsuduki THKNIITM. Long-Term Intake of Fish Oil Increases Oxidative Stress and Decreases Lifespan in Senescence-Cccelerated Mice. Elsevier [Internet]. 2011 [cited 2023 Sep 6]; Available from: https://www.sciencedirect.com/science/article/abs/pii/S0899900710001759
20) Septiana SI, Puruhita N. EFFECT OF GIVING ANchovies (Engraulis encrasicolus) ON SPATIAL MEMORY OF SPRAGUE DAWLEY RATS AGED ONE MONTH. Journal of Nutrition College. 2016;4(1):8–13.
21) Hendrawati A. Protective Effect of Quercetin and Omega-3 Combination on Pancreatic β Cells in Type 2 Diabetes Mellitus Rats. Pharmaciana. 2017 May;7.
22) Lord Yesi. The Effect of Omega-3 Fatty Acid Supplements and Vitamin E on Placental Apoptosis MARKER Levels in White Mice (Rattus Norvegicus) Pre-Eclampsia Model. 2021.2016;5.
Volume 06 Issue 09 September 2023
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