1Dina Utari Almi, 2Hadi Sarosa, 3Setyo Trisnadi
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v7-i02-48Google Scholar Download Pdf
ABSTRACT
Excessive UVB exposure causes health problems in humans, the effect on the skin is molecular and cellular changes. Sunburn is the most obvious acute clinical effect, resulting in the production of TNF-α by keratinocytes, IL-1 is a cytokine that influences TNF-α. Skin wounds release various damage associated molecular patterns (DAMPs) which will activate inflammatory signaling pathways such as NLRP3 inflammasomes and toll-like receptors (TLRs) which cause increased production of inflammatory cytokines. The aim of this study was to examine the anti-inflammatory effect of Manilkara zapota leaf extract which was assessed based on the expression of IL-1 and TNF-α in Wistar rats exposed to UVB. Post-test only control group design experimental research using rats (Rattus norvegicus) as research objects, divided into 4 groups, namely (K1) control rats without treatment, (K2) rats exposed to UVB light then given placebo cream, (K3) rat group exposed to UVB light then given 25% sapodilla leaf extract cream, and (K4) mice were exposed to UVB light then given 50% sapodilla leaf extract cream. The average expression of IL-1 using the One-Way ANOVA test was 0.197 (p0.05), which means there was no significant difference in the average expression of IL-1 between groups. Meanwhile, the average TNF-α expression between groups was 0.036 (p0.05), the results showed that there was a significant difference in the average TNF-α expression between groups, TNF-α expression using the Post Hoc LSD test of sapodilla leaf extract cream 50% dose (K4) reduced TNF-α expression to a lesser extent than K1 or K2. Administration of leaf extract cream (Manilkara zapota) affected the expression of TNF-α, but did not affect the expression of IL-1 in a mouse model experiencing sunburn.
KEYWORDS:sunburn, Manilkara zapota cream, IL-6, TNF-α
REFERENCES1) Alfredsson L, Armstrong BK, Allan Butterfield D, et al. Insufficient sun exposure has become a real public health problem. Int J Environ Res Public Health. 2020;17(14):1-15. doi:10.3390/ijerph17145014.
2) Katsubo: Bizu rabi. 2012-Haru. Entaburein; 2012.
3) Xiao T, Yan Z, Xiao S, Xia Y. Proinflammatory cytokines regulate epidermal stem cells in wound epithelialization. Stem Cell Res Ther. 2020;11(1). doi:10.1186/s13287-020-01755-y.
4) Islam S, Alam MB, Ann HJ, Park JH, Lee SH, Kim S. Metabolite profiling of manilkara zapota l. Leaves by high-resolution mass spectrometry coupled with esi and apci and in vitro antioxidant activity, α-glucosidase, and elastase inhibition assays. Int J Mol Sci. 2021;22(1):1-17. doi:10.3390/ijms22010132.
5) Ramos A, Alvarez MR, Delica K, et al. Antioxidant and Anticancer Activities of Manilkara zapota and Lansium domesticum Leaves Coupled with Metabolomics analysis using Molecular Networking. Vietnam Journal of Chemistry. 2022;60(5):578-588. doi:10.1002/vjch.202100110.
6) Mohd Tamsir N, Mohd Esa N, Omar SNC, Shafie NH. Manilkara zapota (L.) P. Royen: Potential source of natural antioxidants. Malaysian Journal of Medicine and Health Sciences. 2020;16(8):196-204.
7) Alsareii SA, Alzerwi NAN, Alasmari MY, et al. Manilkara zapota L. extract topical ointment application to skin wounds in rats speeds up the healing process. Front Pharmacol. 2023;14(June):1-15. doi:10.3389/fphar.2023.1206438.
8) Puspitasari AD, Yuita NE, Sumantri S. KRIM ANTIOKSIDAN EKSTRAK ETANOL DAUN KOPI ARABIKA (Coffea Arabica). Jurnal Ilmiah Teknosains. 2017;3(2). doi:10.26877/jitek.v3i2.1884.
9) Hernández-Bolio, Dzul-Romero, Velázquez, Cresencio, Hernández-Núñez, Aguirre-Crespo. The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves. Metabolites. 2019;9(10):217. doi:10.3390/metabo9100217.
10) Sharma MR, Mitrani R, Werth VP. Effect of TNFα blockade on UVB-induced inflammatory cell migration and collagen loss in mice. J Photochem Photobiol B. 2020;213. doi:10.1016/j. jphotobiol.2020.112072.
11) Chiu HW, Chen CH, Chen YJ, Hsu YH. Far-infrared suppresses skin photoaging in ultraviolet B-exposed fibroblasts and hairless mice. PLoS One. 2017;12(3). doi:10.1371/journal.pone.0174042.
12) Lee KJ, Park KH, Hahn JH. Alleviation of ultraviolet-B radiation-induced photoaging by a TNFR antagonistic peptide, TNFR2-SKE. Mol Cells. 2019;42(2):151-160. doi:10.14348/molcells. 2018.0423.
13) Malik A, Kanneganti TD. Function and regulation of IL-1α in inflammatory diseases and cancer. Immunol Rev. 2018;281(1):124-137. doi:10.1111/imr.12615.
14) Ansary TM, Hossain MR, Kamiya K, Komine M, Ohtsuki M. Inflammatory molecules associated with ultraviolet radiation‐mediated skin aging. Int J Mol Sci. 2021;22(8). doi:10.3390/ijms22083974.
15) Nasti TH, Timares L. Inflammasome activation of IL-1 family mediators in response to cutaneous photodamage. In: Photochemistry and Photobiology. Vol 88. ; 2012:1111-1125. doi:10.1111/j.1751-1097.2012.01182.x.
16) Shah H, Rawal Mahajan S. Photoaging: New insights into its stimulators, complications, biochemical changes and therapeutic interventions. Biomedicine and Aging Pathology. 2013;3(3):161-169. doi:10.1016/j.biomag.2013.05.003.
17) Weber A, Wasiliew P, Kracht M. Interleukin-1 (IL-1) pathway. Sci Signal. 2010;3(105). doi:10.1126/scisignal.3105cm1.
Volume 07 Issue 02 February 2024
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