1Sukanto, 2Yani Corvianindya Rahayu, 3Herniyati, 4Happy Harmono, 5Raikhan Mahadi
1Department of Pediatric Dentistry, Faculty of Dentistry, University of Jember, Jember, Indonesia
2Department of Oral Biology, Faculty of Dentistry, University of Jember, Jember, Indonesia
3Department of Orthodontics, Faculty of Dentistry, University of Jember, Jember, Indonesia
4Department of Dental Biomedical, Faculty of Dentistry, University of Jember, Jember, Indonesia
5Faculty of Dentistry, University of Jember, Jember, Indonesia
DOI : https://doi.org/10.47191/ijmra/v7-i02-47Google Scholar Download Pdf
ABSTRACT
Periodontal disease is an inflammatory disease of the oral cavity which characterized by inflammation of the gingiva. Theobroma cacao L. is a herbal plant that has antibacterial and anti-inflammatory effects, which is starting to be widely researched as an alternative therapy for inflammation in the oral cavity. The aim of this study was to examined the effect of the ethanol extract gel of cocoa pod husk (Theobroma cacao L.) on increasing the number of fibroblasts and the collagen fiber density in the gingiva of rats that have been induced by Phorphyromonas gingivalis. The sample used 24 male Wistar rats divided into 3 groups. Each group was induced by porphyromonas gingivalis every 3 days for 14 days then anointed with placebo gel (negative control group), metronidazole gel (positive control group), and cocoa pod ethanol extract gel (CPH gel) for 7 and 14 days. Gingival tissue was taken after euthanasia, after which histological preparations were made, and fibroblasts were observed under a microscope and analyzed using quantitative pathology 0.3.2 software, while for collagen density analized using Adobe Photoshop CS6 software. The results showed there was a significant difference of the number of fibroblasts between each group (p<0.05). however there was no significant difference between positive control and cocoa pod husk extract at day 14 (p > 0.05).The treatment group on day 7 and 14 was higher than the other groups. It was conclude that the ethanol extract of cacao pod husk (Theobroma cacao L.) potential to increase the number of fibroblasts and collagen fibers density in gingival tissue of periodontitis rats.
KEYWORDS:gingival fibroblast, collagen fibers, cocoa pod husk, Theobroma cacao L
REFERENCES1) Abnaof K, Mallela N, Walenda G. TGF-β stimulation in human and murine cells reveals commonly affected biological processes and pathways at transcription level. BMC Syst Biol. 2014. 8: 55.
2) Atiqah AN, Poetri AR, Niam MH. The difference of effectivity between mangosteen peel extract and metronidazole on fibroblast proliferation. Odonto: Dental Journal. 2021; 8(1) : 80-85.
3) Baek KJ, Ji S, Kim YC, Choi Y. Association of the invasion ability of Porphyromonas gingivalis with the severity of periodontitis. Virulence. 2015; 6(3), 274-281.
4) Cialdai F, Risaliti C, Monici M. Role of fibroblasts in wound healing and tissue remodeling on Earth and in space. Front Bioeng Biotechnol. 2022 Oct 4;10:958381.
5) Diller RB, Tabor AJ. The Role of the Extracellular Matrix (ECM) in Wound Healing: A Review. Biomimetics (Basel). 2022 Jul 1;7(3):87.
6) Luthfi M, Juliastuti WS, Risky YA, Wijayanti EH, Rachmawati AE, Asyhari, NPO. Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel. Infectious disease reports. 2020; 12(1) : 40-43.
7) Mercandetti M, Cohen A. 2002. Wound Healing and Repair. E Medicine.
8) Nazir MA. Prevalence of periodontal disease, Its association with systemic diseases and prevention. Int J Health Sci (Qassim). 2017; 1(2):72-80.
9) Nugroho SW, Rukmo M, Prasetyo EA, Yuanita, T. Antibakteri ekstrak kulit buah kakao (Theobroma cacao) Streptococcus sanguinis. Conservative Dentistry Journal. 2019; 9(1), 19–21.
10) aRahayu YC, Dharmayanti AWS, Larasati AT. The effect of proanthocyanidin cocoa pod rind extract (Theobroma cacao L.) on MMP-8 expession in gingival tissue of periodontitis rats model. Denta Jurnal Kedokteran Gigi. 2020; 14, 88-93.
11) bRahayu YC, Dharmayanti AWS, Putri YE, Irmawati A. The analysis of proanthocyanidins cacao peel extract (Theobroma cacao L.) potential on the expression of tnf-α and cox-2 on periodontitis rat. Int. J. Pharmaceut. Res. 2020; 2, 4337-43.
12) cRahayu YC, Yuwono B, Wandansari A. The Effectiveness of proanthocyanidins cacao pods (theobroma cacao l) on increasing socket epithelial thickness post tooth extraction in wistar rats. Journal of Dentomaxillofacial Science. 2020; 5(1): 12-16.
13) Rahayu YC, Setiawatie EM, Rahayu RP, Ramadan DE. Analysis of antioxidant and antibacterial activity of cocoa pod husk extract (Theobroma cacao L.). Dental Journal. 2023; 56(4):220-225.
14) Samaranayake, L.h. 2012. Essential Microbiology for Denstistry. Churchill Livingstone: Elsevier Limited.
15) Schilrreff P, Alexiev U. Chronic inflammation in non-healing skin wounds and promising natural bioactive compounds treatment. Int J Mol Sci. 2022 Apr 28;23(9):4928.
16) Smith PC, Martin ez C, Martin ez J, Mc Culloch CA. Role of fibroblast populations in periodontal wound healing and tissue remodeling. Frontiers in physiology. 2019; 10(270): 1-11.
17) Tonetti M S, Jepsen S, Jin L, Otomo-Corgel J. Impact of the global burden of periodontal diseases on health, nutrition and wellbeing of mankind: A call for global action. Journal of clinical periodontology. 2017. https://doi.org/10.1111/jcpe.12732.
Volume 07 Issue 02 February 2024
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