1Nofrina Dwi Perwita Arindani, 2Titiek Sumarawati, 3Prasetyowati Subchan
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v7-i02-44Google Scholar Download Pdf
ABSTRACT
UV radiation causes skin disorders that start from the formation of reactive oxygen species (ROS) and the synthesis of MMP-1 by dermal fibroblasts which plays a role in skin aging, resulting in an increase in IL-6 levels which correlates with tissue damage and inflammation that occurs. The compounds contained in soybean extract are known to act as anti-inflammatory and antioxidants which can reduce inflammation and reduce the negative impact of UV rays on the skin. This study aims to determine the effect of giving soybean extract cream on MMP-1 levels and IL-6 levels in the skin tissue of bablb/c mice induced by UVB light. This research design was a posttest only control group with a completely randomized design method. The samples studied were 36 female balb/c mice with UVB light induction with a wavelength of 302 nm and an energy of 1 MED 5 times a week for 2 weeks. This research was carried out in five groups, namely the healthy group (K1), negative control group (K2), positive control group (K3), with treatment group 1 (K4) with 10% soybean extract cream and treatment group 2 (K5) with extract cream. soybeans 20%. MMP-1 levels and IL-6 levels were analyzed using ELISA. ELISA analysis showed that there was a significant decrease in the mean MMP-1 concentration between K2 (6488 ± 805) compared to K5 (5396 ± 522) with a p value 0.05. Analysis of IL-6 concentration also showed that there was a significant decrease in the mean IL-6 concentration in K4 (270±50) and k5 (242±54) compared to K2 (661±90) with a p value 0.05. Giving soybean extract cream can reduce MMP-1 and IL-6 levels in female Balb/c mice induced by UVB light.
KEYWORDS:UVB light, soybean extract, MMP-1, IL-6
REFERENCES1) Sharma MR, Werth B, Werth VP. Animal models of acute photodamage: Comparisons of anatomic, cellular and molecular responses in C57BL/6J, SKH1 and Balb/c mice. Photochem Photobiol. Published online 2011. doi:10.1111/j.1751-1097.2011.00911.x
2) Kim DJ, Iwasaki A, Chien AL, Kang S. UVB-mediated DNA damage induces matrix metalloproteinases to promote photoaging in an AhR- and SP1-dependent manner. JCI Insight. Published online 2022. doi:10.1172/jci.insight.156344
3) Morgan MJ, Liu ZG. Crosstalk of reactive oxygen species and NF-κB signaling. Cell Res. Published online 2011. doi:10.1038/cr.2010.178
4) Ansary TM, Hossain MR, Kamiya K, Komine M, Ohtsuki M. Inflammatory molecules associated with ultraviolet radiation‐mediated skin aging. Int J Mol Sci. 2021;22(8). doi:10.3390/ijms22083974
5) C. S, T.H. T, N. A, D. L. Ultraviolet-b induced plasmacytoid dendritic cell migration and type 1 interferon signaling in the skin. Arthritis Rheum. Published online 2013.
6) Clydesdale GJ, Dandie GW, Muller HK. Ultraviolet light induced injury: Immunological and inflammatory effects. Immunol Cell Biol. Published online 2001. doi:10.1046/j.1440-1711.2001.01047.x
7) Pang D, Yang C, Luo Q, et al. Soy isoflavones improve the oxidative stress induced hypothalamic inflammation and apoptosis in high fat diet-induced obese male mice through PGC1-alpha pathway. Aging (Albany NY). Published online 2020. doi:10.18632/aging.103197
8) Vera M, Torramade-Moix S, Martin-Rodriguez S, et al. Antioxidant and anti-inflammatory strategies based on the potentiation of glutathione peroxidase activity prevent endothelial dysfunction in chronic kidney disease. Cell Physiol Biochem. Published online 2018. doi:10.1159/000495540
9) Kim IS. Current perspectives on the beneficial effects of soybean isoflavones and their metabolites for humans. Antioxidants. Published online 2021. doi:10.3390/antiox10071064
10) Goh YX, Jalil J, Lam KW, Husain K, Premakumar CM. Genistein: A Review on its Anti-Inflammatory Properties. Front Pharmacol. Published online 2022. doi:10.3389/fphar.2022.820969
11) Miao ZY, Xia X, Che L, Song YT. Genistein attenuates brain damage induced by transient cerebral ischemia through up-regulation of Nrf2 expression in ovariectomized rats. Neurol Res. Published online 2018. doi:10.1080/01616412.2018.1462879
12) Sharma S, Malhotra L, Yadav P, Mishra V, Sharma RS, Abdul Samath E. Genistein: A novel inhibitor of IL-6/IL-6R interface of the Interleukin-6–mediated STAT3 dependent pathway of carcinogenesis. J Mol Struct. Published online 2022. doi:10.1016/j.molstruc.2022.132668
13) Cheng WX, Huang H, Chen JH, et al. Genistein inhibits angiogenesis developed during rheumatoid arthritis through the IL-6/JAK2/STAT3/VEGF signalling pathway. J Orthop Transl. Published online 2020. doi:10.1016/j.jot.2019.07.007
14) Liu X, Liu K, Qin J, et al. C/EBPβ promotes angiogenesis through secretion of IL-6, which is inhibited by genistein, in EGFRvIII-positive glioblastoma. Int J Cancer. Published online 2015. doi:10.1002/ijc.29319
15) Lim TG, Kim JE, Lee SY, et al. The daidzein metabolite, 6,7,4’-Trihydroxyisoflavone, is a novel inhibitor of PKCα in suppressing solar UV-induced matrix metalloproteinase 1. Int J Mol Sci. Published online 2014. doi:10.3390/ijms151121419
Volume 07 Issue 02 February 2024
There is an Open Access article, distributed under the term of the Creative Commons Attribution – Non Commercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/), which permits remixing, adapting and building upon the work for non-commercial use, provided the original work is properly cited.
Our Services and Policies
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected.
The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.
International Journal of Multidisciplinary Research and Analysis will publish 12 monthly online issues per year,IJMRA publishes articles as soon as the final copy-edited version is approved. IJMRA publishes articles and review papers of all subjects area.
Open access is a mechanism by which research outputs are distributed online, Hybrid open access journals, contain a mixture of open access articles and closed access articles.
International Journal of Multidisciplinary Research and Analysis initiate a call for research paper for Volume 07 Issue 11 (November 2024).
PUBLICATION DATES:
1) Last Date of Submission : 26 November 2024 .
2) Article published within a week.
3) Submit Article : editor@ijmra.in or Online
Why with us
1 : IJMRA only accepts original and high quality research and technical papers.
2 : Paper will publish immediately in current issue after registration.
3 : Authors can download their full papers at any time with digital certificate.
The Editors reserve the right to reject papers without sending them out for review.
Authors should prepare their manuscripts according to the instructions given in the authors' guidelines. Manuscripts which do not conform to the format and style of the Journal may be returned to the authors for revision or rejected. The Journal reserves the right to make any further formal changes and language corrections necessary in a manuscript accepted for publication so that it conforms to the formatting requirements of the Journal.