1Ihdina Hanifa Hasanal Ibrahim,2Agung Putra,3Titiek Sumarawati
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2,3Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v6-i9-75Google Scholar Download Pdf
ABSTRACT:
Diabetes mellitus (DM) type 2 is a metabolic disorder with the occurrence of insulin receptor resistance and reduced ability of pancreatic β-cells to secrete insulin, there will be an increase in adipose tissue which causes hypertrophy and hyperplasia. This situation triggers inflammation involving the role of pro-inflammatory cytokines and chemokines such as TNF-α (Tumor Necrosis Factor-Alpha) and MCP-1 (Monocyte Chemotactic Protein-1). Cell-based therapies such as the administration of hypoxic mesenchymal stem cell secretom offer a new paradigm in the management of T2DM. This study aims to determine the effect of hypoxic mesenchymal stem cell (SSPM) administration on TNF-α and MCP-1 gene expression in STZ-induced obese DM rats. This research is experimental with a post-test-only control group design approach. The subjects of the study were 20 male Wistar rats which were randomly divided into 4 groups. Group K1 was given standard feed and distilled water. Group K2 subjects with obesity mellitus type 2 with 0.9% NaCl. Groups K3 and K4, subjects with type 2 obesity mellitus, were treated with secretom doses of 250 μl and 500 μl. The study was conducted at the Sultan Agung Stem Cell Research Institute Laboratory for 21 days by taking blood for examination of TNF-α and MCP-1 gene expression using the PCR method. The result showed that the lowest average TNF expression was in the K4 group (3.19 pg/ml) and the lowest mean MCP-1 expression was in the K4 group (1.57 %). One-way ANOVA test showed a significant difference in TNF- expression with a value of p=0.006 and MCP1 expression showed a significant difference p=0.000 (p<0.05). The Administration of hypoxic mesenchymal stem cell (SSPM) secretom significantly reduced TNF-α and MCP-1 gene expression in STZ-induced obese DM rats.
KEYWORDS:Diabetes mellitus, TNF-α, MCP-1, SPPM
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Volume 06 Issue 09 September 2023
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