1A’an Haries Pranowo, 2Danis Pertiwi, 2Agung Putra
1Postgraduate student of Biomedical Sciences, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
2Stem Cell and Cancer Research, Faculty of Medicine, Universitas Islam Sultan Agung, Jl Kaligawe KM 4 Semarang 50012
DOI : https://doi.org/10.47191/ijmra/v6-i10-44Google Scholar Download Pdf
ABSTRACT:
The autoreactivity of T cells in DMT1 patients causes activation of M1 and M2 macrophages as proinflammatory and anti-inflammatory markers which promote pancreatic cell damage. Secretome Hypoxic Mesenchymal Stem Cells (SH-MSCs) therapy methods play a role in repairing damaged tissue and regenerating pancreatic islet cells. This study aims to examine the effect of SH-MSCs on macrophage cell polarization through the expression of CD68 as a marker of M1 macrophages and CD163 as a marker of M2 macrophages in DMT1 rats. The sample of this study were 5 Wistar rats which were divided into 4 groups; normal group (no treatment), negative control group, and 2 treatment groups with 0.5cc of SH-MSCs (P1 group) and 1cc of SH-MSCs (P2 group). STZ 60 mg/kgBW induced DMT1 in the negative control, P1, and P2 groups. Data were tested parametrically using SPSS 22. The mean of CD68 expression in normal group was 3.06±0.35%, negative control: 21.67±0.93%, P1: 10.98±0.68%, P2: 5.85±0.55%. The mean of CD163 expression in normal group: 22.83±0.51%, negative control group: 3.56±0.50%, P1:15.81±1.80%, P2: 20.80±2.24%. The results of the normality and homogeneity test were p> 0.05. Anova test results p < 0.05. Post Hoc LSD test results p < 0.05. The administration of SH-MSCS was shown to decrease CD68 and increase the CD163 expression significantly in DMT1 rats.
KEYWORD:CD68, CD163, M1 macrophages, M2 macrophages, DMT1
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Volume 06 Issue 10 October 2023
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